DESIGN
image of nanocar
DESIGN OF BODY
DESIGN OF BODY
In red circle, a stiring(M13) is connecting the shaft and the wheel.
This is the only joint between wheel and shaft.
The length of a string is about 10nm(30 base).
SIMULATION
Each of these caDNAno designs were tested in CanDo[1][2] to visualise how thermal motion or how
the fixing the wheel and shaft by staples with azobenzene moieties in the design above would affect the behaviour of the structure. The examined models are 1. Fixing, 2. Not fixing.
1. Fixed : The wheel and shaft is fixed by staple strands with azobenzene moieties.
2. Not fixed : The staple strands are dissociated by cis-trans isomerization of the azobenzene.
About Body
Reference
[1] : DN Kim, F Kilchherr, H Dietz, M Bathe. Quantitative prediction of 3D solution shape and flexibility of nucleic acid nanostructures. Nucleic Acids Research, 40(7):2862-2868 (2012).
[2] : CE Castro, F Kilchherr, DN Kim, EL Shiao, T Wauer, P Wortmann, M Bathe, H Dietz. A primer to scaffolded DNA origami. Nature Methods, 8: 221-229 (2011).